Thursday, December 4, 2008
advance biotech
DNA finger printing-
It is the identityfication of DNA sample by utilizing the particular DNA marker present of the DNA and known as mini satellite. The no. of mini sateliteis different in different sample .This difference is known as VNTR.
Satellite DNA-It is the part of DNA present on non transcribed region having repetitive sequence.
Procedure-1.A drop of blood or serum or a root of hair or virginal swap or vaginal epithelium are mainly used for the DNA finger printing.
2.DNA is extritd from the sample and is subjected to the restricted endonuclease which cuts DNA fairly frequently but not with in mini starlight.
3.These cut fragment having different different length are known as RFLP.
4.The resulting RFLPs are then electrophoresed at gel and these are blotted at nitrocellulose.
5.The minisatalight sequences present at the fragment are detected by the labled DNA probe.
6 After the hybridization of mini satelight to X-ray film.On which minisatelight region develop particular type of bands. These bands are known as DNA finger print,which are unique to each person.
The similarities and dissimilarities of these band help in the identification of person
It is the identityfication of DNA sample by utilizing the particular DNA marker present of the DNA and known as mini satellite. The no. of mini sateliteis different in different sample .This difference is known as VNTR.
Satellite DNA-It is the part of DNA present on non transcribed region having repetitive sequence.
Procedure-1.A drop of blood or serum or a root of hair or virginal swap or vaginal epithelium are mainly used for the DNA finger printing.
2.DNA is extritd from the sample and is subjected to the restricted endonuclease which cuts DNA fairly frequently but not with in mini starlight.
3.These cut fragment having different different length are known as RFLP.
4.The resulting RFLPs are then electrophoresed at gel and these are blotted at nitrocellulose.
5.The minisatalight sequences present at the fragment are detected by the labled DNA probe.
6 After the hybridization of mini satelight to X-ray film.On which minisatelight region develop particular type of bands. These bands are known as DNA finger print,which are unique to each person.
The similarities and dissimilarities of these band help in the identification of person